Manual of Basic Techniques for a Health Laboratory,
World Health Organization
The book is divided into three parts. The first describes the setting-up of a peripheral health laboratory and general laboratory procedures, including use of a microscope and laboratory balances, centrifugation, measurement and dispensing of liquids, and cleaning, disinfection and sterilization of laboratory equipment. Methods of disposal of laboratory waste, dispatch of specimens to reference laboratories and laboratory safety are also discussed. The second part describes techniques for examining different specimens for helminths, protozoa, bacteria and fungi. Techniques for the preparation, fixation and staining of smears are also discussed. The third and final part describes the examination of urine, cerebrospinal fluid and blood, including techniques based on immunological and serological principles. For each technique, a list of materials and reagents is given, followed by a detailed description of the method and the results of microscopic examination.