Monday, December 21, 2009

Methods in Molecular Biology, vol.611 - Histology Protocols 2009

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Histology Protocols 2009
Methods in Molecular Biology, vol.611
Tim D. Hewitson, Ian A. Darby
ISBN 978-1-60327-344-2
e-ISBN 978-1-60327-345-9
Humana Press


Part-I of the book concentrates on the preparation of tissue, providing an overview of fixation, embedding, and processing (Chapter 1), and in Chapters 2 and 3, the required techniques for the retrieval of RNA from histological sections. Both routine and specialist histological staining techniques are provided in Part-II. These include proto- cols for immuno (Chapters 4–7), lectin (Chapter 8), and hybridization (Chapter 9) histochemistry, histological staining (Chapters10and 11), as well as specific methods for the in situ identification of hypoxia (Chapter 12) and apoptosis (Chapter 13). Finally, Part-III details advances in imaging (Chapters 14–16) and image analysis (Chapter 17).

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